necrostatin 1  (MedChemExpress)

Journal: Science Advances

Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination

doi: 10.1126/sciadv.aee4577

Figure Lengend Snippet: ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.

Article Snippet: The pharmacological inhibitors VX-765, Ac-DEVD-CHO, and Nec-1 were purchased from MedChemExpress (USA).

Techniques: Control, Staining, Fluorescence, Microscopy, Flow Cytometry, Immunofluorescence, Western Blot, Analysis, Activity Assay, Expressing, Immune Cell, Two Tailed Test

Journal: iScience

Article Title: Disulfidptosis in PCOS pathogenesis: Multi-omics identification of LRPPRC as a diagnostic biomarker and therapeutic target

doi: 10.1016/j.isci.2026.116561

Figure Lengend Snippet: Direct validation of SLC7A11 expression and exclusionary pharmacological assessment of cell death in PCOS granulosa cells (A) Representative immunofluorescence (IF) staining images showing the expression and localization of SLC7A11 (red) in the ovary of a DHEA-induced PCOS mouse. Nuclei were counterstained with DAPI (blue). White boxes highlight the specific enrichment of SLC7A11 in the granulosa cell layer of the follicles. Magnification: 100×. Images are representative of n = 6 individual mice per experimental group. Scale bars, 100 μm. (B) western blot analysis of SLC7A11 protein expression levels in primary mouse granulosa cells (mGCs) isolated from three independent DHEA-induced PCOS mice (DHEA 1#, 2#, 3#). Tubulin was used as the internal loading control. (C) Flow cytometry analysis (upper panels) and corresponding quantitative bar graph (lower panel) of the cell death rate (PI-positive percentage). LRPPRC-knockdown KGN cells (Sh LRPPRC) were cultured under glucose-sufficient (+Glc) or glucose-deprived (-Glc) conditions, and subsequently treated with or without specific cell death inhibitors: DFO (ferroptosis inhibitor), Z-VAD-FMK (pan-caspase apoptosis inhibitor), or Nec-1 (necroptosis inhibitor). Data are presented as the mean ± SD of n = 3 independent biological replicates. ∗∗∗ p < 0.001 compared with the Sh LRPPRC+Glc group; ∗ p < 0.05, ns = not significant compared with the Sh LRPPRC-Glc group.

Article Snippet: Necrostatin-1 (Nec-1) , MedChemExpress , Cat# HY-15760.

Techniques: Biomarker Discovery, Expressing, Immunofluorescence, Staining, Western Blot, Isolation, Control, Flow Cytometry, Knockdown, Cell Culture